in vitro transcription

Artemia in vitro Transcription System and the Detection of Transcriptional Activity

卤虫体外转录模型及其转录活性检测

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After in vitro transcription, the cleavage reaction was shown to have cut off 79.3% target RNA in 1h.

经体外转录出核酶RZ1及靶RNA后，体外切割反应1小时，79.3%的靶RNA即被切开。

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Northern blot analysis indicates that the 3 ′ terminal non-viral sequence including the ribozyme had been cleaved by the cis-hairpin ribozyme from the in vitro transcripts soon after the in vitro transcription reaction.

Northern杂交结果表明，包括核酶在内的3′末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。

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The relationship between template activity and structural variation of recombinant plasmid DNA was studied in a cell-free system for in vitro transcription which was developed with maize RNA polymerase B and recombinant plasmid DNA.

本文以重组质粒pZmS51DNA为转录模板，在无细胞离体转录系统中研究了质粒DNA的构型变化与其模板活性的关系。

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Aim To study the interference role of Bcl-2 siRNA on HL-60 cells. Methods Bcl-2 siRNA was synthesized in vitro transcription with silencer siRNA construction kit.

目的应用RNA干扰技术观察Bcl2siRNA对白血病细胞HL60中bcl2基因表达的影响，探讨siRNA技术在白血病治疗中的作用。

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